how to dispose of ph buffer solution

Do exercise 17.2.3. /XObject << How to dispose For example, it is known that acidic buffer solutions can be kept for longer than alkaline solutions. << If the total volume of waste to be disposed is greater than four liters per day, approval by OCRS is required. /Kids [4 0 R 5 0 R 6 0 R 7 0 R] stream endobj /MediaBox [.00 .00 612.00 792.00] Buffer solutions and other solutions containing ethidium bromide or acrylamide in any concentration and ethidium I just got a new pH meter with some fisher scientific buffer solution. Post your questions about chemistry, whether they're school related or just out of general interest. /Kids [10 0 R] There are typically 2 types of pH calibration methods, i.e. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". Inorganic acids and bases that have pH 59 (provided that no other regulated chemical is present); Aqueous buffer solutions lacking any other regulated materials and biohazards (e.g. If you are calibrating fairly infrequently, you may wish to consider using single-use solution sachets rather than bottled. WebDispose of the inactivated toxin solution down the drain with a large volume of water. Lets use hypoidous acid, pKa = 10.5, \[[hypoiodite]=[hypoiodus \: acid] 10^{pH-pK_a} \\ [IO^-]=[0.10]10^{10-10.5}=0.032M\]. Waste is considered Contaminated with Chemotherapeutics if disposable items containresidual amounts of substances used to imitate a biochemical response in tissue culture or in animals, including: antineoplastic agents (e.g., cisplatin, doxorubicin, cyclophosphamide); hormones or hormone-like drugs (e.g., estrogens, tamoxifen); synthetic analogs and other carcinogens (e.g., BrdU). Buffer solution pH calculations (video) | Khan Academy 12 0 obj endobj WebContaminated packaging Dispose of as unused product. Chemical Waste Old, outdated, and off spec chemicals need to be disposed of properly. WebFor example it is known that acidic buffer solutions can be kept longer than alkaline ones since carbon dioxide from air may dissolve in the liquid and change the concentration of In general: We guarantee that a solution in a freshly opened bottle meets its specifications provided that is still within its expiry date. << /Resources <<>> >> /Parent 4 0 R Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. endobj Acetic acid has a KA of 1.75x10-5, which gives it a pKA of 4.76. 607-255-8200, or send your question toaskEHS. Accessibility StatementFor more information contact us atinfo@libretexts.org. 900001. /Type /Pages The cookies is used to store the user consent for the cookies in the category "Necessary". >> 7y\ B{o`3X&lPd_\=dl^J;xDO8]Y]VI^Sv5:&D?d!O=lTB\WhOl3Qe$8e7EQ!>;nx> MUTymV:avkQhlIi2i'(SO.y=_A vzw6i_*{%7jGtPq4OYB94N9bbe+> Ul. When calibrating with different buffer solutions, be very careful not to contaminate the buffers. << /9a6bb218-6cd4-410c-8dba-774f34278dad 33 0 R << /Contents [16 0 R 17 0 R 18 0 R] Insert pH probe in cup, wait for reading to settle and calibrate zero point (i.e. /BBox [.00 .00 816.00 1056.00] The blood pH in the range of 7.45 to 7.35 is considered healthy, but outside of this range causes medical problems. Lastly, Biological Waste Guide | Environment, Health and Safety >> WebInorganic buffers (phosphate or bicarbonate based) Buffer solutions containing ethidium bromide that have been filtered, decontaminated, or destroyed. The ideal pH or pOH for a buffer to work at is the pK value of the acid or base that makes the buffer. +86'mCD-[r`MCs*F;RAia?}P4X|:h5xOZ'CC 2#oNS")$Lb=^Q|D{+x#T"_X(I_Z-qCx-fn 8Tl8ZSb r)Io! `Hkm!0o VQ8 Cd7qy]- 1|(\!A0BSz p3qv_W. /Resources << /Filter /FlateDecode >> Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. Single junction reference electrode fill solution, equitransferent solution with Ag/AgCl, 5 x 60 mL. endobj x^+ r r It is recommended to write the pH value on the cap of each bottle to prevent them getting mixed up. When calibrating a pH controller, it is best to calibrate pH7 first and then to calibrate pH4 or pH10. /Kids [8 0 R] x^+ r r >> Guide to Laboratory Sink/Sewer Disposal of Wastes /Author (mthurston) /Length 175 Prudent Practices for Handling Hazardous Chemicals in Laboratories, National Academy Press, Washington, D.C., 1981. Note: Protect drains from chemical spills do not use sinks for chemical storage or secondary containment. << /Title (Error) Last Updated: October 15, 2022 9:40:19 PM PDT. /Type /Pages The Phosphoric acid in its pure form then it can be disposed of directly as it acts as a good fertilizer. /Keywords () >> /Contents [24 0 R 25 0 R 26 0 R] 2(Dual) Point Calibration means the probe is calibrated at a zero point and a slope point. << Literature: White Papers, Guides, Brochures. stream 3 0 obj endobj Please do not post entire problem sets or questions that you haven't attempted to answer yourself. /Type /Pages x^ R R << How to properly dispose of pH calibration solutions : Phosphate Buffer stream 6 0 obj The cookie is used to store the user consent for the cookies in the category "Other. If so, then disposal is usually down the drain with water. Yes, the pH 4, 7, and 10 buffers I checked out the MSDS for each of them and it specifically says not to dispose in the drain. I just checked the Fisher Red 4.00 SDS and I see what you mean! >> WebThe blood maintains its pH of ~7.4 primarily by the carbonic acid/hydrogen carbonate buffer system. Trichloromethyl benzene (benzotrichloride). Details of the supplier of the safety data sheet Company /704b99ad-c15f-4c4f-a669-e52a95deec01 23 0 R endobj >> /Type /Page Legal. Always keep your pH electrode moist. Keep in suitable containers for disposal. endobj Note: Since the buffer was at a higher pH than Ka, the acetate concentration (conjugate base) is greater than the acetic acid concentration. WebNon-Hazardous Substances Permitted for Drain Disposal: Non-toxic common salt solutions (sodium chloride, magnesium chloride, potassium chloride) Biological buffers with pH between 5.5 and 10 (phosphate buffers, TRIS, TAE, etc.) /Resources << 10 0 obj >> Materials characterization with our comprehensive portfolio of instruments, powerful software and a wide selection of accessories and crucibles. 8 0 obj << /Parent 2 0 R Consult any permits (APHIS, PPQ, CDC, etc. stream /Kids [11 0 R] << Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. If also infectious, refer to Biohazard column. Also, a log must record the date, quantity and chemicals being disposed. >> This list contains examples of specific chemicals and does NOT include all chemicals with limited discharge to the sewer. /MediaBox [.00 .00 612.00 792.00] pHuture pH electrode fill solution, 5 x 60 mL. The buffer capacity deals with how much of an external acid or base can be neutralized so effectively that the pH does not change, and this deals with the concentrations of the acid and salt in the logarithmic term of the Henderson Hasselbach equation. << SAFETY DATA SHEET (SDS) Phosphate Buffer 1211 Medical Center Drive, Nashville, TN 37232 pH4). /Parent 2 0 R Buffer solution pH 7.00 V. GHS 2 / 29.11.2017 Print Date 29.11.2017 Page 2/8 Signal Word-Hazard Statements None. endobj I am a bit confused as to what I should do instead. How can you easily dispose of these? /d41783cf-9c7f-4ae9-8a27-ea37b55ff6bd 27 0 R However, this method is not that accurate. pH buffer solutions should always be stored in a cool dry place and should be discarded responsibly if any remains after the expiry date, which is printed on the bottle. Use table 16.3.2 to get the Kb value of propylamine. /Type /Pages For the above two buffers, there was more acid than conjugate base, and so they had a greater capacitance towards an external base than an external acid. << I have never used pH buffer and am pretty new to MSDS sheets. /Count 1 From table 16.3.2 the pKb value for propylamine is 3.46, and a solution with a pH of 10 has a pOH of 4, so this is a reasonable choice. >> This list contains examples of specific chemicals and does NOT include all chemicals that are forbidden from sewer disposal. Prudent Practices for Disposal of Chemicals from Laboratories, National Academy Press, Washington, D.C., 1983. Store and dispose of nonadjusted waste as. /CreationDate (D:20140806153100) hbbd``b`z$gX@e;`A/@Dp+iS\@BmH./ [3 Dilution to bringthe alcohol concentration below 24% for disposal purposes is not allowed. /20563bed-8773-429e-a5b5-58be6438cc3a 19 0 R The following wastes mustNEVERbe discharged to the sanitary sewer in ANY concentration. Shelf /Parent 2 0 R Occasionally, liquid waste is autoclaved. $0.50molNaOH)\left (\frac{1molNaC_2H_3O_2}{1molNaOH}\right)=0.50molNaC_2H_3O_2$, Final mols excess reagent (acetic acid) after complete consumption of limiting reagent Waste is considered Contaminated with Other Biologicals if the contents are not infectious to humans or animals, and are not considered r/sNA. What would be the acetate concentration if the acetic acid concentration is 0.10M? The osmolarity and { "17.01:_Common_Ion_Effect" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17.02:__Controlling_pH-_Buffer_Solutions" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17.03:_Acid-Base_Titrations" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17.04:__Solubility_of_Salts" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17.05:__Precipitation_Reactions" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17.06:_Equilibria_Involving_Complex_Ions" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17.07:__Solubility_and_Complex_Ions" : "property get [Map 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"15:_Equilibria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "16:_Acids_and_Bases" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17:_Aqueous_Equilibria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "18:_Entropy_and_Free_Energy" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "19:_Electron_Transfer_Reactions" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "20:_Coordination_Chemistry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "21:_Nuclear_Chemistry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Appendix_1:_Google_Sheets" : "property get [Map 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\newcommand{\Argument}{\mathrm{Arg}}$ $ \newcommand{\norm}[1]{\| #1 \|}$ $ \newcommand{\inner}[2]{\langle #1, #2 \rangle}$ $ \newcommand{\Span}{\mathrm{span}}$ $\newcommand{\id}{\mathrm{id}}$ $ \newcommand{\Span}{\mathrm{span}}$ $ \newcommand{\kernel}{\mathrm{null}\,}$ $ \newcommand{\range}{\mathrm{range}\,}$ $ \newcommand{\RealPart}{\mathrm{Re}}$ $ \newcommand{\ImaginaryPart}{\mathrm{Im}}$ $ \newcommand{\Argument}{\mathrm{Arg}}$ $ \newcommand{\norm}[1]{\| #1 \|}$ $ \newcommand{\inner}[2]{\langle #1, #2 \rangle}$ $ \newcommand{\Span}{\mathrm{span}}$$\newcommand{\AA}{\unicode[.8,0]{x212B}}$, Buffer Type I: A Weak acid and its conjugate base, Buffer Type II: A Weak base its conjugate acid. /XObject << Disposal of Laboratory Wastes: Requirements for UC San Diego 9500 Gilman Dr. La Jolla, CA 92093 (858) 534-2230, Autoclaving Guidelines for Biohazardous Waste, How to Package and Dispose of Biohazardous and Medical Waste, Controlled Substances: Managing Inventory, How to Store and Dispose of Radioactive Waste, How to Store and Dispose of Hazardous Chemical Waste, Known Hazardous and Extremely Hazardous Waste, How to Dispose of Photo and X-ray Processor Waste, Managing Photo and X-ray Processor Equipment. note, we often omit the spectator ions (sodium) in these equations, and are implying that A- is the anion of a salt. /6944648e-d7f0-4a5e-90dd-d46f63f6dabd 32 0 R /Type /Page /Filter /FlateDecode Single point calibration is usually done with the pH probe in the process water and the user calibrates the instrument to force the controller to read the same as the users independent test results. x^}A E$a6]-@&]SD%QP`W&MM 3?o&NpiHKAz41%B}(,#9A-Ya00zQ{_-""em=>="/_aRss"xPi)n>]JJ! This kind of waste is always considered hazardous chemical waste. The 3 most common pH buffer solutions are pH4, pH7 and pH10, and are usually a different colour to clearly distinguish between them. /MediaBox [.00 .00 612.00 792.00] >> Growth media solutions that have been rendered non-infectious, including culture << You mean the pH 4.00, 7.00, and 10.00 aqueous buffers? /Parent 5 0 R 9 0 obj +l "%;N8NN~:'j{Bzh#qy> \R/[W\E 9>.6y endstream Waste is considered Contaminated with Chemicals if disposable items haveresidual amounts of non-acutely toxic chemicals only (e.g., phenol, chloroform, acrylamide, xylene). So the strategy for picking a buffer is choose an acid with a pKa close to the desired pH and then adjust the salt to acid ratio until they equal. Even if you added water and it did not react, the concentration would go down. 5 0 obj Note: The table of acid ionization constants used to solve16.3.1 problem 17.2.3 had both Ka and pKa values, and if you are looking for an acid to make a buffer, looking at the pKa is much easier. A best practice is to make azide waste solutions basic >pH 10 before The higher the buffer's pH ( from > 7 ), the quicker it will degrade. WebHow do you dispose of your pH buffer. Scan this QR code to download the app now. Uses advised against Food, drug, pesticide or biocidal product use. Sewer discharges of these chemicals must not be prohibited for any other reason. /Type /Pages endobj (from 1.0 mol dm^-3 to 0.5 mol dm^-3). /Resources << /XObject << >> The following chemicals must not be discharged to the sanitary sewer in any concentration. Always contact a permitted waste Single Junction Reference Electrode. FJ+z.\'OH~N.F.%Ovyz^}22RA{SSz*f* WebMineral acids and bases should be neutralized to a pH 5.5 to 9.0 rangebefore disposal see the recommended procedures inAppendix A. Organics Materials listed below in quantities up to about 100g or 100 ml at a time are suitablefordisposal down the drain while flushing with excess water. Cool the liquid to below 150F before pouring it down the drain. 900002. Please note that application of some regulatory requirements to laboratory waste streams is extremely complicated. 2 0 obj /Kids [8 0 R] /Filter /FlateDecode No solids, sludges, or viscous substances, Will not interfere with sewage treatment operations, No hot (150F or higher) temperatures in volumes of more than 10 gallons, If you can't find a particular substance and aren't sure how to dispose of it, contact the. Convenience - saves time, more efficient calibration, avoids waste and spillage. These cookies track visitors across websites and collect information to provide customized ads. One can then rearrange and solve for pH with the Henderson Hasselbach eq. ~]>b?e. \[pH=pK_a+\textcolor{red}{\cancel{\log \dfrac{[salt]}{acid}}} \label{17.2.9}\], This can can also be seen by Equation \ref{17.2.8} where [A-] = [HA] and, \[K_{a}=\dfrac{[H^+]_e \textcolor{red}{\cancel{[A^-]_i}}}{ \textcolor{red}{\cancel{[HA]_i}}} \label{17.2.10}\]. Decant a small amount of pH7 buffer into separate cup. Coordinate with animal facility manager, especially with large animal carcasses. Waste is considered Contaminated with a Biohazard if it contains or is potentially contaminated with human infectious agents, viral vectors used with human and animal cell culture, biologically-derived toxins, human blood and body fluids, all human and animal cell cultures, or fluids and tissues from infected animals. Separate carcasses and tissues from other disposable items (e.g., plastic and paper) whenever possible. Add 3.394 g of Sodium Phosphate Monobasic Monohydrate to the solution. /Parent 7 0 R Serological pipettes can puncture bags when randomly mixed with other disposable items in plastic biohazard bags. )D ?rHwvRLkk\].~NJ6( 50A( %%EOF A solution that is made by diluting 0.5 mol of acetic acid and 0.5 mol of /Length 173 Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors. >> 15 0 obj ex: \[HA + NaOH \rightarrow NaA + H_2O\]. 7 0 obj Do not add bleach in this case. We also use third-party cookies that help us analyze and understand how you use this website. /Type /XObject /MediaBox [.00 .00 612.00 792.00] Find information in the operating manuals, the pH guide booklet and in the pH TroubleShooter on. << Vanderbilt University is required to comply with sewer disposal restrictions established by the Metro wastewater treatment plant and all applicable State and Federal regulations. This website uses cookies to improve your experience while you navigate through the website. endstream << ou8 >> /Type /XObject Double junction reference electrode inner fill solution, equitransferent solution with Ag/AgCl, 5 x 60 mL. This cookie is set by GDPR Cookie Consent plugin. pH7). /Count 1 Always contact a permitted waste disposer (TSD) to assure compliance. /MediaBox [.00 .00 612.00 792.00] Note: When mixing solutions of acids and bases the concentration of the species are being changed by two different factors, chemical reaction and diluting. /Length 9 Yes, the pH 4, 7, and 10 buffers I checked out the Treatment is defined as using a material to neutralize hazardous waste. << /Pages 2 0 R Some exceptions may occur, and will be dealt with on a case by case basis. You must neutralize solutions with a pH outside the range 5.5 to 9.5 before disposal. WebpH buffer solutions should always be stored in a cool dry place and should be discarded responsibly if any remains after the expiry date, which is printed on the The concept of buffer capacity is the same for weak bases and their conjugate acids, except that you use pOH and pKb in the equations, and sometimes those get tricky as you convert them back to pH. common salt solutions and cell culture media). 10 0 obj The according material safety datasheets are available atPRO Certificate Search. Other chemicals may be disposed of in the sanitary sewer only with written email permission from EHS. An acid/base conjugate pair fulfills these requirements: Thus there are two types of buffers, Adding a Base converts HA to A-. WebMineral acids and bases should be neutralized to a pH 5.5 to 9.0 rangebefore disposal see the recommended procedures inAppendix A. Organics Materials listed below in %PDF-1.4 Deactivate with bleach (1 part bleach to 9 parts liquid waste) for 30 minutes before pouring it down the drain. /ProcSet [/PDF /ImageB /ImageC /ImageI /Text] This cookie is set by GDPR Cookie Consent plugin. 15 0 obj EH&S will collect, process and dispose of your old hazardous chemicals. DO NOT store electrode in distilled or deionized water this will cause ions to leach out of the glass bulb and render your electrode useless. A buffer is a solution that maintains a constant pH when an external acid or base is added to it. /Type /Pages All rights reserved. WebSection 13 Disposal Information Disposal Methods: Dispose in accordance with all applicable Federal, State and Local regulations. However, as we have no means of knowing how a solution is stored once the bottle has been opened we cannot guarantee for the quality of the solution after that. OH /Title (Error) endstream WebPhosphate Buffer (pH 7.2) was developed to provide a standardized medium for the preparation of sample dilutions. These cookies ensure basic functionalities and security features of the website, anonymously. /Count 1 1597 0 obj <>stream /Type /Page What do you do with your pH buffer? /Type /Catalog Lets consider an acid and its salt, but realize we could apply this to a base and its salt. /Count 1 /Contents [16 0 R 17 0 R 18 0 R] NO, unless in volumes of less than 10 gallons. /Type /Page Also note, if, \[[salt]>[acid] \; then \; \frac{[salt]}{[acid]}>1\; and\; log\frac{[salt]}{[acid]} \text{is a positive number and pH is greater than pK}_a \\ [salt]<[acid] \; then \; \frac{[salt]}{[acid]}<1\; and \; log\frac{[salt]}{[acid]} \text {is a negative number and pH is less than pK}_a \], Note, like any logarithm, you can write this in the log form, on in the power form. endstream endobj startxref endobj /Author (mthurston) 0 9 0 obj endobj The pH must be above 3 and below 9. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. We are committed to an inclusive environment where everyone has the chance to thrive and to the principles of equal opportunity and affirmative action. Dispose of substances such as automotive oil, gear oil, and machinery grease as. HANDLING AND STORAGE Precautions to be taken in handling and storage: Avoid contact with eyes, skin and clothing. endobj UC San Diego 9500 Gilman Dr. La Jolla, CA 92093 (858) 534-2230 For higher concentrations: For concentrated, unused processor chemicals and other spent solutions: Some chemicals are hazardous in any concentration. Drain Disposal Guide Bundle the serological pipettes into a plastic sleeve conveniently placed inside the biohazard bag, which organizes them and prevents them from puncturing the outer red biohazard bag. << Dilution of higher concentrations of any alcohol is, NO, unless the concentration is nonhazardous and does not contain methanol or other hazardous chemicals. WebUnwanted dye stock solutions, spent running buffer solutions and destaining solutions that contain the dyes can either be collected and disposed of through the HWMU or collected and run through an approved filter device. Whatever goes down the drain ends up in the ocean, so the San Diego Metropolitan Wastewater Department strictly regulates sewer waste. WebProduct Identifier: Trade Name: Phosphate Buffer, pH 6.0, pH 9.1 or custom Chemical Name: Phosphate Buffer Catalog Number: pH 6.0 14006381, pH 9.1 14000049 Kit Number: Use of chemical: Buffer Identification of Manufacturer: Manufacturer/Supplier: Biochemical Diagnostics, Inc. You may be held liable for violations of applicable laws. 4 0 obj All user manuals come with necessary information about the short and long time storage of the respective probe. The breadth, depth and veracity of this work is the responsibility of Robert E. Belford, rebelford@ualr.edu. (615) 322-5000, Making Health Care Personal Initial moles sodium hydroxide = 0.5000(1.0) = 0.50 mol, Final mols sodium acetate formed after complete consumption of limiting reagent (sodium hydroxide) /Count 1 note, we often omit the sodium (spectator ion) in these equations, \[B+H_2O \leftrightharpoons BH^+ + OH^-\], ex: \[HCl + NH_3 \rightarrow NH_4^+ +Cl^-\], Note, we often omit the sodium (spectator ion) in these equations, What is the concentration of a solution made by neutralizing 500.0 ml of 2.0M acetic acid with 500.0 ml 1.0M sodium hydroxide, assume the volume of the solutions are additive (500ml + 500ml = 1,000ml), \[HC_2H_3O_2 + NaOH \rightarrow NaC_2H_3O_2 + H_2O\], Initial moles acetic acid = 0.5000(2.0) = 1.0 mol For example it is known that acidic buffer solutions can be kept longer than alkaline ones since carbon dioxide from air may dissolve in the liquid and change the concentration of protons towards lower pH values. A test certificate of all calibration solutions and many electrolytes can be downloaded by visiting Buffer. 216.368.2000 >> endobj Add 20.214 g of Sodium Phosphate Dibasic Heptahydrate to the solution. Drain Disposal Guide - UGA Dilution of higher concentrations is not allowed. Web610001.
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how to dispose of ph buffer solution 2023